M1 Development of Drugs and Health Products

Dr D. Bonte, Dr E. Morel and Prof. V. Rosilio Prof. I. Kansau B. Trimbach.

Cohesion week French classes Videos - oral presentations Rallyes in Paris, serious games and interaction with French people Mooc for the visit of Paris-Saclay and the Faculty of Pharmacy

Updates in biotechnology: - discussed decision of a research topic in biotechnology among student group. - preparation of a final talk through several rounds of challenging questions (several aspects should be considered: Science, society, economy, clinical trial, etc).

Cultural activities to promote interactions between students from different origins.
Basic notions of French language.
Team building through the frame of a red thread with a final biotech defense in front of a jury. Best presentation will be rewarded by a talk at the end of the year during the student symposium.

Being registered to the M1 master's degree D2HP.

Superdupont, Lob, Tome 1, Fluide Glacial, 2008.

Octobre.

CHATENAY

Co coordinator: Pr C. POÜS Dr A. BAILLET, Dr B. BENOIT, Dr N. CHAREF, Dr A. PILON, and Pr C. POÜS.

Cellular and molecular mechanisms of tumorigenesis: -Introduction: cancer types and stages, cell signaling, targets for therapy, personalized medicine (0h30) -Cell cycle, proliferation, DNA repair, immortalization, cellular senescence, checkpoints (2h) -Histones, post-translational modifications, epigenetics (1h30) -Mitosis, microtubules (1h) -Cell death: apoptosis, necrosis and others. Autophagy. Resistance to death (2h) -Metabolism, angiogenesis, inflammation, tumoral microenvironment (3h) -EMT, polarity, migration, metastasis, cancer stem cells (3h)

ASSESSMENT: 1st session: written exam (70%), oral presentation of article analysis (30%) 2nd session: written exam.

The objectives of this course is to :
- Settle the basic knowledge in oncology necessary to understand the cell biology of cancer, new curative strategies and scientific / vulgarized literature in the field of cancer
- Highlight the main pathologies and origins encountered in cancer
- Improve the knowledge of mechanisms and fundamental basis of cancer development
- Understand current and under investigation therapeutic strategies used in cancer
- Perform a critical analysis of bibliographic experimental data related to cancer
- Be able to identify the exploration methods of cancer cell biology and therapy.

Be enrolled in the international master’s degree D2HP.

Robert A. Weinberg, The Biology of Cancer, Garland Science, 2013.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Co-coordinator Dr L De Chaisemartin Dr F. Adam, Dr E. Bianchini, Pr D. Borgel, Dr C. Caseri, Dr L. de Chaisemartin, Pr S. Chollet-Martin, Dr O. Christophe, Pr C. Denis, Dr A. Gleizes, Dr V. Granger, Dr D. Helley, Dr A. Kauskot, Dr P. Lenting, Dr V. Marin-Esteban, Dr M. Peyneau, Dr V. Picard, Dr F. Saller, and Dr A. Stepanian.

A - Immunology(20h): Basic knowledge in immunology (e-learning) - Auto-immune diseases - Allergic diseases - Graft and alloimmunity - Immune deficiencies - Monoclonal gammopathies B- Hematology (20h): - Integrative approach to hemostasis: hemostasis overview, physiology of primary hemostasis, coagulation and fibrinolysis. - Exploration of hemostasis - Routine diagnostic tests: exploration of primary hemostasis, exploration of coagulation. - Pathologies of primary hemostasis: thrombocytopenia and platelets dysfunction, Von Willebrand factor, related diseases, diagnostic and treatments. - Hemophilia: replacement therapies and resistance, innovative treatments in heamophilia (now and future).

To develop students’ knowledge in immunology and associated diseases, hemostasis, coagulopathies and thrombosis.

To be enrolled in the M1 "Development of Drugs and Health Products".

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Dr J.P. Benitah, Pr D. David, Dr-MD S. Edhery, Dr R. Fischmeister, Pr A. Gardier, Dr A.-M. Gomez, Dr C. Lemaire, Dr J. Leroy, Dr B. Manoury, Pr J.J. Mercadier, Dr M. Merickskay, Dr D. Mika, Dr E. Morel, L Pereira, Dr J Piquereau, Pr V. Vecksler, and Dr R. Ventura-Clapier.

-Introduction to heart physiopathology, hypertrophy and heart failure, EC coupling and arrhythmias, technics of cardiac investigation, renin-angiotensin system with MR/GR regulation, cardiac energetics, vascular system and associated pathology, pharmacolo.

This teaching unit will set up and develop students’ knowledge in the main major functions of human physiology with focus on cardiology, respiratory function and neurophysiology. Students will thus develop knowledge of the general principles and parameters of physiology, functions and will get a short introduction to dysregulation and associated pathologies.
Student’s knowledge will be improved on classical regulations and molecular signaling involved in each of these major functions in order to setup a shared basic knowledge necessary to handle the pathological part with associated therapies, in M2.
Students will gain knowledge of the models used in physiology in order to perform quantitative and qualitative analyzes (clinical parameters and biomarkers)
-Students will acquire critical analysis of bibliographic experimental data related to physiology, organ function, and signaling pathways.

Being enrolled in the international master’s degree D2HP.

- Robbins and Cotran, Pathologic Basis of Disease, 9th Edition, Elsevier 2019 - Michael Zigmond Joseph Coyle Lewis Rowland, Neurobiology of Brain Disorders,1st Edition, Academic Press 2015 - Neil Herring, David J. Paterson, Levick's, Introduction to Cardi.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Co coordinator: Dr E Morel Dr G Bernadat, Dr D. Bonte, Dr V. Boudy, Pr N. Chaput, Dr F. Faye, Dr E. Morel, Dr P. Paolantonacci, and Dr I. Turbica.

Upstream process I Applied molecular biology, bioproduction, upscale processes in pharmaceutics, development of a candidate drug from bench to bedside, vectorisation I Downstream process Bioprocesses, recombinant proteins, biosimilars, comparison of different recombinant proteins of interest (production, purification, QC) Advanced biotechnology I New technics in biotechnology, new targets in therapy I: 1/ identification and validation of new molecules, 2/ preclinical and clinical steps, new medicine (conception and development), introduction to gene and cell therapy Conferences Technologies to produce medicinal products derived from human blood Regulatory framework for biotherapeutics I Tutorials: oral presentations.

The objectives of this courses are:
-Introduction to the basics of biotechnology applied to health products with all the aspects ranging from molecular conception and vectorisation, bioproduction to specific purification processes of recombinant proteins. In addition, students will have access to the most advanced and up to date technics in biotechnology, a necessity in this rapidly evolving field.
-Thorough knowledge of the methods and strategies used to prepare gene transfer (vectors, targeting and host production selection) in order to produce current and future potential recombinant therapeutic proteins
-Critical analysis of literature documents
-Development of adaptation capacity with new technics and mechanisms used in biotechnology (CRISPR-Cas9), personal medicine, gene and cell therapy
-Relational, collaborative, and communication skills (written and oral).

Being registered in the international master’s degree D2HP.

Daan J. A. Crommelin, Robert D. Sindelar, Bernd Meibohm. Pharmaceutical Biotechnology: Fundamentals and Applications, Springer, 5th ed. 2019.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Florence AGNELY Kawthar BOUCHEMAL Nicolas HUANG François-Xavier LEGRAND Ali MAKKY Jean-Philippe MICHEL Gilles PONCHEL Veronique ROSILIO Claire SMADJA Mariana VARNA + Invited professionals from the industry (specific topics) + invited French or foreign academic lecturers (specific themes).

Theory, Case study.

The objectives are to introduce students to the basics of biopharmacy, and to preformulation and formulation applied to small chemical molecules and proteins, as well as the processes used to manufacture conventional drug forms.
- Biopharmacy of small molecules and peptides / proteins
- Physicochemical bases for the formulation of small molecules and proteins
- Conventional pharmaceutical forms (tablets, injectables, gels, emulsions)
- Case study.

To be enrolled in the M1 Development of drugs and health products.

- G.L. Amidon et al. A theoretical bases for Biopharmaceutic drug classification, Pharm. Res 1995 - R. Ghadi, N. Dand, BCS class IV drugs: Highly notorious candidates for formulation development, J. Control Rel. 2017 - L. Hovgaard, et., Pharmaceutical For.

Octobre - Novembre - Décembre.

CHATENAY

A. Kasselouri co-coordinator of the teaching unit C. Aymes-Chodur , P. Chaminade , T. Duc Mai , D. Libong , I. Lepotier , R. Michael-Jubeli , J.-P. Michel , S. Tfaili , A. Tfayli , T. Tran-Maignan.

Theory and tutorials.

Different spectral and separative techniques and their different contributions in the analysis of small molecules up to macromolecules of pharmaceutical and biological interest.The students learn how to justify the choice of a methodology and how to optimize the parameters of separation techniques (separation and detection).

A-Chromatography (GC, HPLC, UHPLC), Capillary electrophoresis and associated detection methods (UV/Fluo/Electrochem/CAD/SM)
B-Spectral methods and analytical imaging: SEM, AFM, IR, RAMAN, near IR, basics of chemometrics for spectral data
C-Thermal analysis: TSC, DSC, TGA.

To be enrolled in the M1 Development of drugs and health products.

- Introduction to Modern Liquid Chromatography, 3rd Edition; Lloyd R. Snyder, Joseph J. Kirkland, John W. Dolan; Wiley, 2011; ISBN: 978-1-118-21039-0 . - Principles of Instrumental Analysis; Douglas A. Skoog, F. James Holler, Stanley R. Crouch; Cengage L.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Co coordinator Dr D Bonte Dr E Morel Mr M Blanc, animator CESIM.

A dedicated Pharma scenario, consisting of four customer segments (Children, Hospitals) in two market areas (Europe, Asia) and an up to six different products with varying features is proposed. Teams of students are competing for the leadership position at the end of play (winning criteria is share price). They play in teams (5-6 students) online against others with the help of serious game instructors. Schedule in 2 phases: A ½ day kick-off session in order to set up a dynamic context for the game with a game instructor. He also presents: -Key learning goals and European context -Serious game scenario, rules and online demo -Game schedule and expected deliverables - a blank training course

A game phase : 7 rounds of about 4 hours each. At the end, a debriefing session will be held with final results announcement. Student’s delivery: a management report, a corporate strategy and assessment of that strategy, an advertisement.

A serious game on Pharmaceutical Marketing is an online management simulation, focused on marketing strategy. It helps students to develop their understanding and command of the whole marketing decision-making process for health products and drugs, from strategy design to sales execution.
Students will develop their understanding of pharmaceutical marketing operations, in a dynamic and competitive environment. They will implement (or learn by doing) core marketing concepts of a health-related product and corporate finance. They will improve their English in specific domains and also their ability to work in a team.
Students will develop:
Ability to work under time constraints
Improvement of marketing and finance knowledge
Critical analysis of technical and financial documents
Critical analysis of their own decisions and consequences all over the rounds
Relational, collaborative, and communication skills during this intensive session.

Being registered in master's degree D2HP Good English proficiency (independent English use, in writing and speaking) Master level or equivalent in Pharmaceutical or Health related studies, with optional basic knowledge in marketing Team-working skills.

Link to website of CESIM www.cesim.com.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Co cordinator: Dr E Morel D Bonte (UPSaclay), Dr S. Fehaili (Sup’Biotech), E Mogensen (Sup’Biotech), Dr E Morel, and Dr I. Turbica (UPSaclay).

Bioreactors Study of the production of the prodigiosine pigment and identification of the best bacterial strain and physiological conditions for production.

A. Presentation of the prodigiosine and its properties B. Presentation/identification of the producing bacteria C. Presentation and taking in charge of the bioreactor Biostat® Aplus (Sartorius) used in clean room manufacturing conditions D. Bacterial growth follow up (over 6h) with controlled parameters. Three conditions are setting up with variation of temperature, oxygen and pH on three different bioreactors led by 3 different student teams E. Practical analysis of potential contamination with characterization of the potential contaminated strain F. Theoretical reminder on bacterial growth and metabolite production and comparison with growth record from the different teams.

The objectives of these practical works are:
-To improve practical and theoretical skills of the students on one of the major technics used in industrial production: the bioreactor
-To give a practical experience of research in clean room and aseptic conditions
Student should acquire the practical skills to manipulate biotechnology products or microorganisms in aseptic conditions
Students should be able to adapt properly protocols to the new working conditions
Students should acquire skills in the domain of recombinant protein production as used in Industries
Students should be able to take into account the practical conditions and constraints of conception/production in their future interaction with industrials
Critical analysis of literature documents
Relational, collaborative, and communication skills (oral and written).

Being registered to the master's degree D2HP.

Alok Barua, Goutam Saha, Satyabroto Sinha, Bioreactors: Animal Cell Culture Control for Bioprocess Engineering, CRC Press 2015.

Septembre - Octobre - Novembre - Décembre.

Co-coordinator Pr C Fernandez Pr I Kansau.

This teaching unit is composed of a 3 day hospital internship. Tutorials explaining the organization of the hospital services will be performed by clinicians Students should find by themselves their internship and are encouraged to find their hospital placements in the focux of interest of the D2HP (hematology, biochemistry, immunology, toxicology, clinical pharmacy, ...) assessment will be a report writing at the end of the internship.

The aim of this teaching unit is to provide students with a first experience in hospital work. Thus, students will discover the organization of hospital services.

Being registered in the M1 master's degree D2HP M1 students without previous hospital experiences.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Caroline MASCRET Sylvain FORGET (Blue Dil).

Theory and project.

Roles and missions of a Marketing department (affiliate-based vs HQ based)
Roles and mission of a Product manager (affiliate-based vs HQ based)
Market analysis, objectives and tools
Strategy tactics and plan of action (definitions & examples)
Execution of the marketing activities
How to deal with pricing issues from a marketing perspective
Main regulatory rules regarding marketing activities.

To be enrolled in the M1 Development of drugs and health products.

Septembre - Octobre - Novembre - Décembre.

CHATENAY

Vincent FAIVRE Ali MAKKY Jean-Philippe MICHEL Mariana VARNA.

Tutorial and practical works of pharmaceutical technology.

The objective of this teaching unit is to provide the students with practical application of the theoretical knowledge related to the formulation, manufacturing and control of conventional drug delivery systems.

Preparation and characterization of emulsions, gels, tablets, and injectable forms.

To be enrolled in the M1 Development of drugs and health products.

Octobre - Novembre - Décembre.

CHATENAY

Co cordinator virology: Dr M Lussignol co coordinator bacteriology: Pr I Kansau co coordinator parasitology: Pr P Loiseau Dr D. Bonte, Dr T. Candela, Pr A. Esclatine, Pr C. Janoir, Pr I. Kansau, Dr C. Larrazet, Pr P. Loiseau, Dr M. Lussignol, Dr J-C Marvaud, and Dr S. Pomel.

A- Virology -Basic knowledge in virology and virus-host interactions (structure, multiplication cycle, diagnosis, pathologies and symptoms) -Viruses worldwide: HIV, hepatitis viruses (HBV, HCV), herpes viruses and influenza viruses - Major human arboviruses and tropical viral diseases Tutorials: presentation of one virus, one pathology B- Bacteriology - Structure of the bacterial surface - Bacterial genetics and gene regulation - Bacterial toxins (mechanisms, regulation) - Bacterial spore - Host response Tutorials: publication analyses C- Parasitology and medical mycology Diversity of parasites, life cycles and environmental risks Vector control Invasive species of ecto/endo parasites and mechanism of antiparasitic drugs Biological diversity of fungi and mechanism of antifungal drugs Tutorials: publication analyses.

The courses and personal work in infectiology will reinforce the basic knowledge of viruses, bacteria, parasites and fungi biology, as well as the host response and the strategies developed for the control of the main infectious diseases.
-Thorough knowledge of the basic knowledge in infectiology (virology, bacteriology, parasitology and mycology) in order to understand the strategies developed to control infectious diseases and to overcome drug resistance
-Understanding of the main issues about the needs to discover new therapeutic molecules, to improve the prevention and the diagnosis
-Critical analysis of literature documents
Relational, collaborative, and communication skills (written and oral).

Being registered in the international master's degree D2HP.

Fields Virology, Lippincott Williams & Wilkins 2015, 6th edition Parasitology (Protozoology and Helminthology) KD Chatterjee, CBS; 13 edition, 2015 Textbook of Medical Mycology, Jagdish Chander,Jaypee Brothers Medical Publishers; Fourth edition, 2018.

Janvier - Février - Mars - Avril.

CHATENAY

M. Beniddir S. Delarue-Cochin E. Drège L. Evanno N. Gigant A. Hamze.

Theory.

Advanced organic chemistry :
A-Introduction: Main strategies of drug discovery (4h)
B-Fundamental approaches for the synthesis of natural and synthetic drugs (20h)
a.C-C bond formation in total synthesis of natural products and drugs
b.C-N and C-O bond formation in total synthesis of natural products and drugs
C-Heterocycles in medicinal and natural product chemistry (8h)
a.Recent advances in multi-component modular synthesis of heterocycles
b.Selective functionalization of heterocycles.

To be enrolled in the M1 Development of Drugs and Health Products.

- Organic Chemistry by J. Clayden, N. Greeves and S. Warren, 2012, OUP Oxford - Applications of Transition Metal Catalysis in Drug Discovery and Development: An Industrial Perspective by M. L. Crawley and B. M. Trost, 2012, John Wiley & Sons, Inc. - Stere.

Janvier - Février - Mars - Avril - Mai - Juin.

CHATENAY

N. Bouaïcha S. Karolak L. Oziol + Invited professionals.

Theory and case studies.

Objectives: Introduce the students to the problem of pollution of the environment by organic molecules (drugs, pesticides, illicit drugs): risk assessment, chemical analysis of residues in environmental matrixes, characterization of pollution.

Content:
A-Residues of drugs in the aquatic environment: methods of analysis, sampling issues. Pollution mapping: Identification of pollution sources, wastewater treatment and abatement, contamination of various media (surface water, groundwater, drinking water) (case study).
B-Evaluation of the ecotoxicological (on fauna) and sanitary (human exposure to residues via food/drinking water/air) risks: prioritization approach, mixed effect issue (case study).
C-Environmental risk assessment for application for marketing authorization (EMEA directive).

To be enrolled in the M1 Development of drugs and health products.

- Guideline on the environmental risk assessment of 4 medicinal products for human use - EMEA/CHMP/SWP/4447/00 Rev. 1 - Guidance for IndustryEnvironmental Assessment of HumanDrug and Biologics Applications - http://www.fda.gov/cder/guidance/index.htm. -.

Janvier - Février - Mars - Avril - Mai - Juin.

CHATENAY

A. Biola-Vidamment, F. Coudoré, D. David, A. Gardier, J.-P. Guilloux, K. Hardonnière, S. Kerdine-Römer, B. Manoury, M. Pallardy, L. Tritschler, and V. Leblais.

Courses, ED, group work, project, demonstrations.

To introduce to the students the general notions in pharmacology and toxicology and the main in vitro and in vivo experimental methods and strategies developed in toxicology, pre-clinical pharmacology (cardiovascular, central nervous system), pharmacokinetics and clinical pharmacology.
A. Generalities: Metabolism, generalities in pharmacology, generalities in toxicology, animal experiments (classical models, transgenic mice), definition of the 3Rs
B. In vivo models:
- Pharmacology models: neurodegenerative pathologies (study methods, parameters, relationships with the clinic), Anxious and depressive disorders (study methods, parameters, relationships with the clinic), cardiovascular models.
- Toxicology models: methodologies in reprotoxicology and immunotoxicology; Carcinogenesis methodologies, methodologies in cardiology.
- Pharmacokinetics
C. In vitro models: development of an alternative method; cell lines, 3D models, chips; cellular models in pharmacology and toxicology; AOP.
D. Tutored project: a 20-page report on the themes addressed.

To be enrolled in the M1 "Development of Drugs and Health Products".

- Casarett & Doull’s Toxicology: The Basic Science of Poisons (Curtis D. Klassen) - Principles and Methods of Toxicology (A. Wallace Hayes, Claire L. Kruger) - The Pharmacological Basis of Therapeutics (Goodman & Gilman, Ed. McGraw-Hill) 2017.

Janvier - Février - Mars - Avril - Mai - Juin - Juillet.

CHATENAY

E.Morel (co-coordinator of the teaching unit) I. Kansau D. Bonte + Invited French and foreign lecturers.

A full day meeting will be organized with scientific topics related to the focus of D2HP (biotechnology, analytics, chemistry, ..) and organized along the already defined axes of the master. Each session will be composed of a specialist of the research domain invited by the students followed by M2 oral presentations. The M1 student will present their lab internship results or their bibliographical work in Poster session. Best poster and oral communication will be rewarded. Students will be trained with theory and tutorials for project management. Oral communication.

The symposium is organized jointly by the students of the master 1 level helped by the master 2. The symposium consists in the presentation of the results of their research project (internship or bibliographical work).
Project management.

Registration to the master D2HP.

Juin - Juillet.

CHATENAY

M. Beniddir E. Drège J. Kaffy P. Le Pogam-Alluard.

Tutorials and practical works.

Objectives: Develop skills for assigning spectroscopic and spectrometric data for structural determination of molecules and macromolecules. Exploit the complementarity of different spectroscopic techniques.

Content:
Fundamentals of chemical spectroscopy and mass spectrometry
Practical works: acquisition and utilization of spectral data for identification of natural and synthetic structures.

To be enrolled in the M1 Development of drugs and health products.

Janvier - Février - Mars - Avril - Mai - Juin.

CHATENAY

Co coordinator: Dr E Mogensen (Sup'Biotech) Dr D Bonte (Univerité Paris-Saclay), Dr E Mogensen (Sup'Biotech), and Dr E Morel (Université Paris-Saclay).

Day 1 1. Preparation of appropriate quantity of culture media and the number of Petri dishes 2. Cloning step with enzymatic digestion of the insert and the vector 3. Electrophoresis in a preparative agarose gel and purification of the 2 fragments to be ligated 4. Ligation reaction Day 2 (half a day) 5. Preparation of competent bacteria and transformation Day 3 (half a day) 6. Selection of transformed colonies and inoculation Day 4 7. Purification of the recombinant plasmid/construction validation and final report writing.

Practical objective:
To produce a strain of E. coli expressing a His-tagged GFP protein. Students will clone the His-tagged GFP-encoding gene into an expression plasmid and integrate it into a strain of E. coli.
Experimental steps are as follow:
-Vector and insert digestion using NheI and EcoRI restriction enzymes
-Insert and vector purification
-Ligation of the insert and vector
-Recombinant plasmid transferred by transformation in bacteria
-The colonies of interest will be grown in liquid medium to allow the amplification of the recombinant plasmid
-After extraction and purification of the plasmid, the success of the cloning technique will be determined.

Technical objectives
- To manipulate in conditions that are appropriate in terms of sterility, temperature, etc…
- To understand and perform a cloning strategy;
- To learn how to search and analyse nucleotide sequences;
- To purify plasmid DNA using a commercial kit and to learn the required analysis methods;
- To prepare, load and run preparative and analytic agarose gels;
- To transform an E. coli strain with plasmid DNA and select the resulting transformants of interest;
- To validate cloning results using molecular biology tools.

Being registered in the master's degree D2HP.

Janvier - Février - Mars - Avril.

CHATENAY

Co coordinator: Dr S. Pomel Dr D. Bonte, Pr A. Esclatine, Pr C. Janoir, Dr M. Lussignol, and Dr S Pomel.

A-Virology Diagnosis of herpesvirus by virus isolation (cell culture, infection with wild-type virus and titration by plaque assay) (4h of practical works + discussion) Diagnosis of cytomegalovirus by qPCR (1h of practical works + 2h of result analysis) Diagnosis of hepatitis B virus by ELISA (3h of practical works + 1h of result discussion)

B-Bacteriology The main principles for the diagnosis of bacterial infections: clinical diagnosis, type of clinical samples, direct bacterial identification by up-to-date methods (mass spectrometry and qPCR), indirect diagnosis (1h of class / tutorials) Presentation of clinical cases by the students (individual or in group depending on the number of students): one clinical case associated with one appropriate diagnostic method with a discussion about the limits of each method (2h of tutorials) C-Parasitology Diagnosis of toxoplasmosis (3h of practical works + 1h of result analysis).

The theoretical part of this unit will give students an update about diagnostic methods used in medical laboratories of virology, bacteriology and parasitology. Diverse items will be presented like the diagnostic techniques, clinical samples result analysis, and limits of the methods.
The practical part will permit the students to manipulate and master different classical diagnostic methods, like viral isolation, qPCR and ELISA.
Students should acquire the theoretical part and the practical skills to perform the diagnosis of various infectious pathogens (viruses, bacteria, and parasites)
Student should be able to manipulate microorganisms and viruses in aseptic conditions
Students should be able to adapt properly protocols
Critical analysis of their experimental results
Critical analysis of literature documents
Relational, collaborative, and communication skills (oral and written).

Being registered in the M1 master's degree D2HP.

Janvier - Février - Mars - Avril.

CHATENAY

Co coordinator: Dr D Bonte Dr E Bianchini, Dr D Bonte, Dr F Fay, Dr M Lussignol, and Dr E Morel.

Students will perform a synthetic bibliographical work in the aim of publishing that work on a specific/accurate scientific topic given by a researcher. This project will last 2 months during the academic year. Each group of M1 students (4-6 individuals) will work on one aspect of the proposed research topic. The coordinator could be a researcher/professor/PI from the University/associate industrial partners (Servier, IRMIT…) and will have to manage, follow one or several groups of students until the expected publication (could be more than 2 months). Student’s team in agreement with their tutor will select the accurate journal (selected impact factor) and will write the associated cover letter. Several groups coudl work on the same topic until publication and all students and supervisor contributing to the publication will be cited on the publication. During the 2 months: 1 introductory course, learning of how to make bibliography research 5 mandatory appointments between the students and the tutor.

Students will be trained to publication work as tool for communication useful in several careers (ranging from engineer to researcher) with the objectives of:
- handling the literature data linked to research topic proposed by the tutor
- improving communication skills and team communication due to early and strong interaction with students team and supervisor
- building and managing a publication project with the overview starting from the background of literature, paragraph and topic selections, innovative points of view, contacts with the publication board and cover letter in agreement with their supervisor
- managing networks and human resources to fulfill the final research publication program
- plan ahead their research project publication: building Gantt and PERT diagrams for each milestones deliverable
- seeking journals for publication with accurate impact factor
- improving oral (exchange) and writing communication all along the training period.

Being registered in the master's degree D2HP.

Mai - Juin.

CHATENAY

Co coordinator: Pr V Rosilio, co coordinator : Dr D Bonte co coordinator : Dr E Morel.

The Lab internship must last 2 months under the direction of a research supervisor. The research work will be the subject of a written report and an oral defense. Part of the research presentation and of the grade could be obtained in the frame of the symposium.

The aim is to complete classical scholar education by a training by research. Students will have to find and perform a lab internship for 2 months. Research subjects and laboratories selected by the student will be validated by D2HP organizing committee.

Being registered to the M1 master's degree D2HP.

Mai - Juin - Juillet.

CHATENAY

B. Trimbach.

Individual and group work for presentation of English communication formats: poster, abstract, oral presentation.

Use Scientific English communication formats: poster, abstract, oral presentation to acquire a scientific and technical vocabulary and develop ability to give a presentation of scientific project work and discuss results in English.

To be enrolled in the M1 "Development of Drugs and Health Products".

Janvier - Février - Mars - Avril - Mai - Juin - Juillet.

CHATENAY