[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-966788","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  Training in Genetics\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          2.5\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semestre 2\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                                      \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux pratiques :\u003C\/span\u003E\n                                      \u003Cspan\u003E30\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                                          \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EVersion Fran\u00e7aise :\u003Cbr\u003E\nConnaissances de base en G\u00e9n\u00e9tique, notamment conjugaison bact\u00e9rienne, transformation bact\u00e9rienne, et notions sur les \u00e9l\u00e9ments g\u00e9n\u00e9tiques mobiles.\u003C\/p\u003E\n\u003Cp\u003EVersion Anglaise :\u003Cbr\u003E\nBasic knowledge in Genetics, including bacterial conjugation, bacterial transformation, and notions on mobile genetic elements.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EProgramme \/ plan \/ contenus\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EVersion Fran\u00e7aise :\u003Cbr\u003E\nCette UE pratique enseign\u00e9e en partie en anglais consiste \u00e0 adopter une d\u00e9marche de g\u00e9n\u00e9tique formelle chez les bact\u00e9ries, en s\u2019appuyant sur un exemple de r\u00e9gulation g\u00e9nique impliqu\u00e9e dans le catabolisme du maltose. Les objectifs sont de caract\u00e9riser ph\u00e9notypiquement des mutants affect\u00e9s dans le r\u00e9gulon maltose, de d\u00e9finir si les mutations engendrent un effet polaire ou non, et d\u2019identifier le g\u00e8ne mut\u00e9 par une approche de cartographie g\u00e9n\u00e9tique, en utilisant la conjugaison bact\u00e9rienne et la compl\u00e9mentation fonctionnelle par transformation bact\u00e9rienne.\u003Cbr\u003E\nCette UE pratique permet d\u2019approfondir sous un angle exp\u00e9rimental, les notions abord\u00e9es dans les Cours et TD de g\u00e9n\u00e9tique bact\u00e9rienne de l\u2019UE \u0022Genetics\u0022.\u003C\/p\u003E\n\u003Cp\u003EEnglish Version :\u003Cbr\u003E\nThis practical course taught partly in English consists in adopting a formal genetic approach in bacteria, using an example of gene regulation involved in the catabolism of maltose. The objectives are to phenotypically characterize affected mutants in the regulation of maltose catabolism, to define whether or not the mutations generate a polar effect, and to identify the mutated gene by a genetic mapping approach, using bacterial conjugation and functional complementation by bacterial transformation.\u003Cbr\u003E\nThis practical course provides an opportunity to explore the concepts covered in the lectures on bacterial genetics in the \u201cGenetics\u201d course, from a more experimental point of view.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EVersion Fran\u00e7aise :\u003Cbr\u003E\n\u00c0 l\u2019issue de cet enseignement, l\u2019\u00e9tudiant sera capable de :\u003Cbr\u003E\nOAV1. D\u00e9finir ce qu\u2019est un op\u00e9ron, un r\u00e9gulon, une mutation polaire et expliquer les cons\u00e9quences d\u2019une telle mutation sur l\u2019expression de g\u00e8nes organis\u00e9s en op\u00e9ron.\u003Cbr\u003E\nOAV2. Expliquer et sch\u00e9matiser les processus de conjugaison bact\u00e9rienne et de compl\u00e9mentation fonctionnelle par transformation.\u003Cbr\u003E\nOAV3. R\u00e9aliser des tests de g\u00e9n\u00e9tique bact\u00e9rienne (par conjugaison et transformation bact\u00e9riennes) pour identifier les g\u00e8nes mut\u00e9s, en choisissant judicieusement le g\u00e9notype des souches\/des plasmides \u00e0 utiliser, en d\u00e9finissant les modes de s\u00e9lection. Concevoir les diff\u00e9rentes \u00e9tapes de mutagen\u00e8se par transposition mises en place pour obtenir des mutants, ainsi que les cribles permettant d\u2019isoler les mutants d\u2019int\u00e9r\u00eat.\u003Cbr\u003E\nOAV4. Proposer des exp\u00e9riences permettant de tester les hypoth\u00e8ses \u00e9mises, analyser les r\u00e9sultats de conjugaison bact\u00e9rienne et de compl\u00e9mentation fonctionnelle par transformation bact\u00e9rienne, les interpr\u00e9ter.\u003Cbr\u003E\nOAV5. Pr\u00e9senter sa d\u00e9marche scientifique et discuter ses r\u00e9sultats de mani\u00e8re critique et argument\u00e9e, en utilisant le vocabulaire adapt\u00e9 en anglais.\u003C\/p\u003E\n\u003Cp\u003EEnglish Version :\u003Cbr\u003E\nAt the end of this course, the student will be able to :\u003Cbr\u003E\nOAV1. Define what is an operon, a regulon, a polar mutation and explain the consequences of such mutation on the expression of genes organized into operons.\u003Cbr\u003E\nOAV2. Explain and illustrate the bacterial conjugation and functional complementation processes by bacterial transformation.\u003Cbr\u003E\nOAV3. Perform bacterial genetic tests (using bacterial conjugation and transformation) to identify mutated genes, carefully choosing the genotype of the strains\/plasmids to be used and defining the selection methods. Design the various steps of transposon mutagenesis used to obtain mutants, as well as the screens used to isolate mutants of interest.\u003Cbr\u003E\nOAV4. Propose experiments to test the hypotheses put forward, analyze the results of bacterial conjugation and functional complementation by bacterial transformation and interpret them.\u003Cbr\u003E\nOAV5. Present its scientific approach and discuss its results critically and in a well-argued manner, using appropriate vocabulary in English.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EOrganisation g\u00e9n\u00e9rale et modalit\u00e9s p\u00e9dagogiques\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EVersion Fran\u00e7aise : \u0026nbsp;\u003Cbr\u003E\nL\u2019enseignement s\u2019articule autour de travaux pratiques qui sont compl\u00e9t\u00e9s par des notions th\u00e9oriques en rapport avec les exp\u00e9riences r\u00e9alis\u00e9es. \u0026nbsp;\u003Cbr\u003E\nEn d\u00e9but de s\u00e9ance, des discussions de groupe en anglais permettent de faire le point sur l\u2019avanc\u00e9e des exp\u00e9rimentations et sur l\u2019analyse des r\u00e9sultats obtenus et d\u2019\u00e9tablir la d\u00e9marche scientifique \u00e0 adopter pour avancer. Les \u00e9tudiants sont ainsi form\u00e9s aux techniques exp\u00e9rimentales et sont mis en situation de \u00ab Recherche \u00bb. Cet exercice permet de former les \u00e9tudiants \u00e0 la communication orale en anglais, et d\u00e9veloppe leur esprit de synth\u00e8se et leur esprit critique.\u003C\/p\u003E\n\u003Cp\u003ELors de la derni\u00e8re s\u00e9ance, les \u00e9tudiants devront \u00e9galement retrouver et proposer le protocole de mutagen\u00e8se et de s\u00e9lection qui a permis d\u2019isoler les mutants qu\u2019ils ont \u00e9tudi\u00e9s, lors de discussions de groupe en anglais.\u003C\/p\u003E\n\u003Cp\u003EEnglish Version : \u0026nbsp;\u003Cbr\u003E\nThe teaching is structured around practical work which is supplemented by theoretical notions related to the experiments carried out.\u003Cbr\u003E\nAt the beginning of each session, group discussions in English aims to evaluate the progress of the experiments and to discuss about the results obtained and to establish the scientific approach to be adopted in order to move forward. The students are thus trained in experimental techniques and are placed in a \u0022Research\u0022 situation. This exercise trains the students in oral communication in English, and develops their synthesis and critical thinking skills.\u003C\/p\u003E\n\u003Cp\u003EDuring the last session, the students will also propose the mutagenesis and selection protocol used to isolate the mutants they studied, during group discussions in English.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                                \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Continue non Int\u00e9grale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]