[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-959354","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  Cell-Free Systems\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          2.5\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semester 1\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ECours magistraux :\u003C\/span\u003E\n                                      \u003Cspan\u003E8\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux pratiques :\u003C\/span\u003E\n                                      \u003Cspan\u003E12\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                                          \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EBasic knowledge of molecular biology\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EProgramme \/ plan \/ contenus\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThis course class is composed of 8h of lecture and 12h of practical work using the MICALIS cell-free biofoundry. The practical work will include the usage of the biofoundry software platform to maximize protein productivity in cell-free.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThe objectives of this course are to provide students with an understanding of practical aspects developed in the laboratory for the study of cell-free systems and, through concrete examples, illustrate experimental methods to increase protein production in cell-free.\u003Cbr\u003E\nAt the end of the course, students will be able to:\u003Cbr\u003E\n- Know the diversity and the different usages of cell-free systems\u0026nbsp;\u003Cbr\u003E\n- Know-how to prepare cell-free systems from cell cultures and cell extracts\u003Cbr\u003E\n- Understand the element required to obtain a functional transcription translation (TX-TL) system.\u003Cbr\u003E\n- Use the automated protocol hosted on the Cell-Free Biofondry at MICALIS\u003Cbr\u003E\n- Know-how to optimise the productivity of cell-free systems\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EOrganisation g\u00e9n\u00e9rale et modalit\u00e9s p\u00e9dagogiques\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003ETeaching in this EU will include 8 hrs of lectures with simple exercises (TD) given during the courses on specific points and 12hrs of practical work (TP). The practical work will consist of expressing a protein of interest using the Cell-Free biofoundries hosted at the Micalis institute.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EBibliographie\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003E- Soudier P, et al. ACS Synthetic Biology, 2022, 11(8):2578-2588 DOI: 10.1021\/acssynbio.2c00138\u0026nbsp;\u003Cbr\u003E\n- Pandi A, et al. Nat Commun., 2022, 13(1):3876. DOI: 10.1038\/s41467-022-31245-z\u003Cbr\u003E\n- Batista AC , et al. Engineering Biology 5 (1), 10-19. DOI: 10.1049\/enb2.12004\u003Cbr\u003E\n- Soudier P, et al. Methods Mol Biol. 2022, 2433:303-323. DOI: 10.1007\/978-1-0716-1998-8_19\u0026nbsp;\u003Cbr\u003E\n- Sabeti Azad M, et al. \u0026nbsp;J. Vis. Exp. 2022, (186), e64236 DOI: 10.3791\/64236\u0026nbsp;\u003Cbr\u003E\n- Pandi A, et al. Nat Commun., 2022, 13(1):3876. DOI: 10.1038\/s41467-022-31245-z\u003Cbr\u003E\n- Borkowski O, et al. Nature Communications, 11(1): 1872, 2020. DOI: 10.1038\/s41467-020-15798-5\u0026nbsp;\u003Cbr\u003E\n- Pandi A, et al. Nature Communications, 10: 3880, 2019. DOI: 1038\/s41467-019-11889-0\u003Cbr\u003E\n- Pandi A, ey al. ACS Synth Biol. 8(8):1952-1957, 2019. DOI: 10.1021\/acssynbio.9b00160\u003Cbr\u003E\n- Voyvodic PL, et al. Nature Communications, 10(1):1697, 2019. DOI: 10.1038\/s41467-019-09722-9\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Continue non Int\u00e9grale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]