[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-959350","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  Chips for Molecular Evolution\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          2.5\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semestre 1\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                                      \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux pratiques :\u003C\/span\u003E\n                                      \u003Cspan\u003E20\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                                          \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003Enone\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EProgramme \/ plan \/ contenus\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003E- Collective debriefing on the purpose of the practical course and the protocol\u003C\/p\u003E\n\u003Cp\u003E- Manipulations: preparing E. coli, pouring, degassing, baking, then peeling off the PDMS chip from the master mold, creating inlet\/outlet, sealing the chip to a coverslip by using plasma treatment, injecting\/spinning cells into chip\u2019s growth channels, preparing tubing and growth medium, flowing the growth medium into the device by using a syringe pump, performing image and data analysis on images previously generated using this type of chip\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThe main pedagogical intention behind this training is to:\u003C\/p\u003E\n\u003Cp\u003E- introduce students to the use of the microfluidic tool for biology research, to highlight its specificities and potentialities.\u003C\/p\u003E\n\u003Cp\u003E- allow students to build\/use on their own a specific microfluidic chip which allows monitoring growth, gene expression or mutation accumulation in single cells of rod-shaped bacteria on long timescales (i.e. few hundreds of generations)\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003EAt the end of the training, students will be able to:\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E- state the differences (advantages and disadvantages) between macrofluidic and microfluidic systems for biology research\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E- define and explain the main steps in the construction of a microfluidic chip\u003C\/p\u003E\n\u003Cp\u003E- build a microfluidic chip in PDMS (from a reusable master mold)\u003C\/p\u003E\n\u003Cp\u003E- perform image and data analysis on images previously generated using this type of chip\u003C\/p\u003E\n\u003Cp\u003E- design, plan a master mold (circuit) modification to facilitate its use in the lab or to address a specific biological question defined by the student\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                                \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EBibliographie\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003E- Taheri-Araghi S, Jun S. In Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods. Springer. 2015, pp 5\u201316\u003C\/p\u003E\n\u003Cp\u003E- Robert L, Ollion J, Robert J, Song X, Matic I, Elez M. Mutation dynamics and fitness effects followed in single cells. Science. 2018, 359:1283-1286.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E- Robert L, Ollion J, Elez M. Real-time visualization of mutations and their fitness effects in single bacteria. Nature Protocols. 2019, doi:10.1038\/s41596-019-0215-x.\u003C\/p\u003E\n\u003Cp\u003E- Ollion J, Elez M, Robert L. High-throughput detection and tracking of cells and intracellular spots in mother machine experiments. Nature Protocols. 2019, doi:10.1038\/s41596-019-0216-9.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E- Elez M, Murray AW, Bi LJ, Zhang XE, Matic I, Radman M. Seeing mutations in living cells. Curr Biol. 2010, 20:1432-1437.\u003C\/p\u003E\n\u003Cp\u003E- Wang P, Robert L, Pelletier J, Dang WL, Taddei F, Wright A, Jun S. Robust growth of Escherichia coli. Curr Biol. 2010, 20:1099-103.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Continue non Int\u00e9grale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]