[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-959342","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  Metabolic Engineering\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          3.5\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semestre 1\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ECours magistraux :\u003C\/span\u003E\n                                      \u003Cspan\u003E12\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                              \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux dirig\u00e9s :\u003C\/span\u003E\n                                      \u003Cspan\u003E8\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                            \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EUnderstanding metabolic pathways and enzymatic reactions is crucial. A background in molecular biology, gene expression, regulation, and recombinant DNA technology is typically required, as well as knowledge of chemical or biochemical engineering principles. \u003Cstrong\u003EA solid understanding of central metabolism and major metabolic pathways is also essential.\u003C\/strong\u003E In addition, the use of computational bioinformatic tools is required in this course.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EProgramme \/ plan \/ contenus\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThe purpose of the metabolic engineering is to generate a cell factory that produces cost-effective molecules at industrial scale.\u0026nbsp;This course aims\u0026nbsp; to provide the basic concepts and tools that have been developed the past 20 years when designing, building and testing metabolic pathways for bioproduction purposes. Practical applications and successes will be surveyed by an industrial (Abolis Biotech). A particular emphasis will be given to two research forefronts of metabolic engineering (1) how can we learn to improve pathway performances using flux analysis and genetic engineering and (2) how pathway engineering can be used to build biosensor and biocomputation devices.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EMetabolic engineering is becoming a mature field of research with many industrial applications.\u0026nbsp;It is a process of optimizing native metabolic pathways and regulatory networks or assembling heterologous metabolic pathways for production of targeted molecules using molecular, genetic and combinatorial approaches.\u003C\/p\u003E\n\u003Cp\u003EAt the end of the course, students will be able to:\u003C\/p\u003E\n\u003Cp\u003E- Explain the various steps and methods necessary to perform a metabolic engineering project\u003C\/p\u003E\n\u003Cp\u003E- Conceive a workflow from design to test in order to produce a given molecule in a given chassis strain\u003C\/p\u003E\n\u003Cp\u003E- Evaluate various methods in order to optimise the production yields of metabolic pathways\u003C\/p\u003E\n\u003Cp\u003E- Adapt metabolic engineering to build biosensor and biocomputing devices\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EOrganisation g\u00e9n\u00e9rale et modalit\u00e9s p\u00e9dagogiques\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThe course module consists of 12 hours of lectures and 8 hours of tutorials introducing the main concepts and workflows of metabolic engineering. Throughout the course, students will apply their knowledge of metabolic engineering, systems biology, and synthetic biology using the Galaxy-SynBioCAD platform (\u003Ca rel=\u0022nofollow noopener noreferrer\u0022 target=\u0022_blank\u0022 href=\u0022https:\/\/galaxy-synbiocad.org\/\u0022\u003Ehttps:\/\/galaxy-synbiocad.org\/\u003C\/a\u003E) to assess the feasibility of producing a heterologous compound in a chassis strain such as \u003Cem\u003EEscherichia coli\u003C\/em\u003E or \u003Cem\u003ESaccharomyces cerevisiae\u003C\/em\u003E.\u003C\/p\u003E\n\u003Cp\u003EAt the end of the course, students will be asked to \u003Cstrong\u003Edesign a metabolic pathway\u003C\/strong\u003E in a given chassis strain, \u003Cstrong\u003Epropose an experimental plan\u003C\/strong\u003E to construct and test the designed pathway, and \u003Cstrong\u003Eevaluate the associated costs\u003C\/strong\u003E, including manpower and consumables.\u003C\/p\u003E\n\u003Cp\u003EThe assessment will be based on two components:\u003C\/p\u003E\n\u003Col\u003E\n\u003Cli\u003EAn oral presentation (15 minutes followed by 5 minutes of questions).\u003C\/li\u003E\n\u003Cli\u003EA short bibliographic report (maximum 1,000 words).\u003C\/li\u003E\n\u003C\/ol\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EBibliographie\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EA textbook is not required in this course. We will use primary literature materials and power point slides. But, students are welcome to do some background reading using recommended reviews below:\u003Cbr\u003E\n- Stephanopoulos GN, Aristidou AA, Nielsen J. Metabolic Engineering: Principles and Methodologies. San Diego: Academic Press 1998.\u003Cbr\u003E\n- Palsson BO. Systems Biology. Cambridge University Press, New York, NY, 2006.\u003Cbr\u003E\n- Smolke CD. The Metabolic Pathway Engineering Handbook, CRC Press (two volumes edited book), 2010.\u0026nbsp;\u003Cbr\u003E\n- Halper R. Systems Metabolic Engineering, Methods in Molecular Biology, Vol. 985, Springer, 2013.\u003Cbr\u003E\n- https:\/\/doi.org\/10.1039\/D0CS00155D\u003Cbr\u003E\n- https:\/\/www.sciencedirect.com\/journal\/metabolic-engineering\u003Cbr\u003E\n- https:\/\/doi.org\/10.1016\/j.ymben.2020.10.005\u0026nbsp;\u003Cbr\u003E\n- https:\/\/doi.org\/10.1016\/j.ymben.2020.08.015\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Continue non Int\u00e9grale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]