[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-956541","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  Single-cell omics and spatial transcriptomics\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          3\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semester 1\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ECours magistraux :\u003C\/span\u003E\n                                      \u003Cspan\u003E25\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                                                        \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003EMolecular biology:\u003C\/strong\u003E understanding of the concepts of gene, DNA\/RNA, transcription, translation, PCR, sequencing, etc.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EGenetics and genomics:\u003C\/strong\u003E knowledge of genome structure, genetic variations, annotation, and principles of high-throughput sequencing (NGS).\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EGeneral bioinformatics:\u003C\/strong\u003E understanding of the principles of analysis pipelines, biological databases, etc. Ability to understand and interpret data generated by high-throughput sequencing.\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EBasic knowledge of statistics applied to biology\u003C\/strong\u003E : tests, data representation, normalization.\u003C\/li\u003E\n\u003C\/ul\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cul\u003E\n\u003Cli\u003EUnderstand the fundamental principles of single-cell omics approaches and their advantages compared to bulk population analyses.\u003C\/li\u003E\n\u003Cli\u003EDescribe the key experimental steps involved in single-cell transcriptomics, including cell isolation, RNA capture, UMI, reverse transcription, amplification, and library preparation.\u003C\/li\u003E\n\u003Cli\u003EIdentify the main technologies and platforms used in single-cell RNA-seq (primarily 10x Genomics) and understand their respective advantages and limitations.\u003C\/li\u003E\n\u003Cli\u003EAcquire the foundations of bioinformatics analysis of single-cell data: quality control, normalization, dimensionality reduction, clustering, and cell-type annotation.\u003C\/li\u003E\n\u003Cli\u003EInterpret biological results obtained from single-cell data to explore cellular heterogeneity, differentiation trajectories, and transcriptional signatures.\u003C\/li\u003E\n\u003Cli\u003EUnderstand the principles and methodology of spatial transcriptomics: tissue fixation, in situ hybridization, spatial transcript capture, and molecular mapping.\u003C\/li\u003E\n\u003Cli\u003EUnderstand the visualization and integrative analysis tools combining single-cell and spatial data (e.g., Seurat, Spatial Transcriptomics Tools).\u003C\/li\u003E\n\u003Cli\u003EAssess the quality and reproducibility of data generated from single-cell and spatial technologies, taking into account technical and biological biases.\u003C\/li\u003E\n\u003Cli\u003EUnderstand and apply Single-Cell Omics and Spatial Transcriptomics approaches to address complex biological questions, particularly in the study of cellular heterogeneity within solid tumors in cancer research and the characterization of different brain regions in neuroscience.\u003C\/li\u003E\n\u003Cli\u003EApply a critical approach when interpreting results from single-cell and spatial analyses, especially in relation to the underlying biological questions.\u003C\/li\u003E\n\u003Cli\u003EDesign and write a research project incorporating Single-Cell Omics and\/or Spatial Transcriptomics methodologies, including scientific objectives, choice of experimental and analytical approaches, and data analysis strategies.\u003C\/li\u003E\n\u003C\/ul\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EOrganisation g\u00e9n\u00e9rale et modalit\u00e9s p\u00e9dagogiques\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EScientific lecture-type conference delivered by specialists in the field of oncology or neurology, heads of sequencing platforms, or bioinformatics engineers specialized in single-cell omics and\/or spatial transcriptomics analyses.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                                \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Terminale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]