[{"command":"insert","method":null,"selector":".course-modal-ajax-placeholder-955501","data":"\u003Cdiv class=\u0022container content-rte\u0022\u003E\n  \u003Cdiv class=\u0022row\u0022\u003E\n    \u003Cdiv class=\u0022box col-md-6 bg-primary text-white\u0022\u003E\n      \u003Ch2 class=\u0022h1 text-left\u0022\u003E  R\u00e9sistance aux antibiotiques: purification et analyse structurale par CryoEM de transporteurs bact\u00e9riens\n\u003C\/h2\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EECTS :\n          6\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n                  \u003Cdiv class=\u0022field-spacing\u0022\u003E\n        \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ESemestre calendaire :\n          Semester 1\n        \u003C\/div\u003E\n      \u003C\/div\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ED\u00e9tail du volume horaire\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ECours magistraux :\u003C\/span\u003E\n                                      \u003Cspan\u003E16\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux pratiques :\u003C\/span\u003E\n                                      \u003Cspan\u003E24\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                          \u003Cspan class=\u0022field__label h4 mt-5 \u0022\u003ETravaux dirig\u00e9s :\u003C\/span\u003E\n                                      \u003Cspan\u003E10\u003C\/span\u003E\n                      \u003C\/div\u003E\n                                                            \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ELangue d\u0026#039;enseignement\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EAnglais\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EEnseignement \u00e0 distance\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003Enon\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n    \u003Cdiv class=\u0022box col-md-6\u0022\u003E\n            \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EPr\u00e9requis\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EEtudiants ayant valid\u00e9 un niveau M1\/ing\u00e9nieurs avec des connaissances en biochimie et chimie des prot\u00e9ines.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EProgramme \/ plan \/ contenus\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003EThe goal of this teaching unit \u0026nbsp;is to offer an hands-on training for master students for the purification of membrane proteins for functionnal and cryoEM structural characterisationing as a model an ABC transporter involved in antibiotic efflux.\u003C\/p\u003E\n\u003Cp\u003EDuring the first week, students will purify a bacterial membrane protein responsible for antibiotic resistance. The purified transporter will then be reconstituted into a lipid environment using nanodiscs, which provide a native-like membrane setting suitable for both functional and structural characterization. Each student pair will work with a distinct lipid composition to assess how membrane composition influences the transporter enzymatic properties, particularly its drug-stimulated ATPase activity. Students will perform a full analysis of their purification results and enzymatic activity measurements.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003EIn parallel, they will attend a series of lectures and seminars given by experts in membrane proteins, antibiotic resistance, biochemistry, and structural biology. These sessions aim to connect the experimental work carried out during practical sessions to a broader molecular understanding of resistance mechanisms and to current strategies for developing new therapeutic approaches. The week will conclude with a student driven scientific discussion, where their will compare, and discuss the collective results obtained by the different groups, reiforcing their scientific communication and collaborative analysis.\u003C\/p\u003E\n\u003Cp\u003EDuring the second week, students will use their purified and reconstituted transporter samples to prepare cryoEM grids. They will learn the practical aspects of grid preparation, vitrification, and data collection using the cryoEM instrumentation at the I2BC. The acquired micrographs will then be processed through all key computational steps, including motion correction, particle picking, 2D classification, 3D reconstruction, and map refinement, to obtain a high-resolution density map of the transporter. Using this map, students will build and refine an atomic model, allowing them to visualize the structural features underlying the protein function and its role in antibiotic resistance. This hands-on experience will be complemented by lectures and workshops focusing on cryoEM data processing workflows leading to cryoEM maps compatible with atomic model building and their analysis.\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EObjectifs d\u0026#039;apprentissage\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cul\u003E\n\u003Cli\u003EOAV 1 : Conna\u00eetre et discuter les m\u00e9canismes mol\u00e9culaires d\u2019antibior\u00e9sistance au travers d\u2019exemples diversifi\u00e9s.\u003Cbr\u003E\nOAV 2 : Conna\u00eetre et discuter les techniques de purification et caract\u00e9risation fonctionnelle des prot\u00e9ines membranaire.\u003C\/li\u003E\n\u003Cli\u003EOAV 3 : Conna\u00eetre et discuter les techniques de pr\u00e9paration d\u2019\u00e9chantillon pour la cryo microscopie \u00e9lectronique.\u003C\/li\u003E\n\u003Cli\u003EOAV 4\u0026nbsp;: Conna\u00eetre et discuter les strat\u00e9gies d\u2019analyse des donn\u00e9es de particules uniques en cryo microscopie \u00e9lectronique.\u003C\/li\u003E\n\u003Cli\u003EOAV 5 : Partager ses r\u00e9sultats avec ses camarades et savoir faire une analyse synth\u00e9tique de l\u2019ensemble des r\u00e9sultats obtenus par le groupe (mise en commune d\u2019une partie des r\u00e9sultats de TP).\u003Cbr\u003E\nOAV 6 : Communiquer \u00e0 l\u2019\u00e9crit et l\u2019oral des r\u00e9sultats scientifiques (compte rendu et pr\u00e9sentation orale de leurs r\u00e9sultats de TP).\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003E\u003Cstrong\u003EComp\u00e9tences \u00e0 acqu\u00e9rir :\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003ECA 1 : Purification de prot\u00e9ine membranaire\u003C\/li\u003E\n\u003Cli\u003ECA 2 : Reconstitution de prot\u00e9ine membranaire en d\u00e9tergent dans un environnement lipidique (nanodisques).\u003C\/li\u003E\n\u003Cli\u003ECA 3 : Pr\u00e9paration d\u2019\u00e9chantillon et traitement de donn\u00e9es issues pour la cryo microscopie \u00e9lectronique.\u003C\/li\u003E\n\u003Cli\u003ECA 4 : Construction et analyse de mod\u00e8le mol\u00e9culaires \u00e0 partir de carte de densit\u00e9 obtenue de carte de densit\u00e9 \u00e9lectronique.\u003C\/li\u003E\n\u003C\/ul\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n        \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003EOrganisation g\u00e9n\u00e9rale et modalit\u00e9s p\u00e9dagogiques\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003E\u003Cp\u003ELieu d\u0027enseignement : Campus Paris-Saclay\u003C\/p\u003E\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n                                \u003Cdiv class=\u0022field-spacing\u0022\u003E\n      \u003Cdiv class=\u0022field__label h3 dash\u0022\u003ENature de l\u0026#039;\u00e9valuation\u003C\/div\u003E\n                        \u003Cdiv class=\u0022mt-5 text-justify\u0022\u003E\n                                      \u003Cspan\u003EEvaluation Continue non Int\u00e9grale\u003C\/span\u003E\n                      \u003C\/div\u003E\n                  \u003C\/div\u003E\n      \u003C\/div\u003E\n  \u003C\/div\u003E\n\u003C\/div\u003E\n","settings":null}]