Le laboratoire applique et développe les méthodes et techniques du génie génétique, de l’analyse structurale, de la bioinformatique et, de manière générale, de l’ingénierie biomacromoléculaire. Il s’intéresse aux bases moléculaires des processus fondamentaux du vivant.
The laboratory uses and develops the methods of genetic engineering, of structural analysis, of bioinformatics and more generally of macromolecular engineering. We are studying the molecular bases of key biological processes.
General themes :
- Structure-function relationships in the translation machinery.
The macromolecules we study insure faithful translation of the genetic message. They are either proteins or ribonucleic acids (RNA).Some of these macromolecules are potential targets for antibacterial agents.
- Selenium toxicity.
Our interest for this element comes from its dual role in mammals.Indeed,selenium is strictly required in trace amounts but becomes toxic if its concentration is too high. Added in food at sub-toxic concentrations, selenium may prevent the appearance of several cancers.
- Cytosqueleton and cell motility.
We strive to understand the role of the actin cytoskeleton in membrane remodeling. The Arp2/3 complex is a molecular machine that creates branched actin networks, which are known to generate a force. This force participates in intracellular traffic and in membrane protrusion during cell migration. In this context, we are looking for novel regulators of the Arp2/3 complex using various screens in order to understand the different roles of branched actin networks in the cell.
- Structural bioinformatics.
We are developing methods for in silico protein engineering using directed evolution. The methods are applied to the design of novel proteins with specific properties, such as binding a particular ligand. We are also working on protein structure prediction.